KOVA
If you are counting microscopic particles - eliminate error at every step
The KOVA System makes it easy, safe and cost efficient to standardise every step of microscopic analysis
Disposable Glasstic slides reduce pipetting errors and eliminate variables that can interfere with microscopic analysis.
Glasstic slides feature self-contained chambers (wells) for precise volume control.
With the unique Glasstic ten-chamber design, getting fantastic results can be cost effective too. Compared to glass slides and cover slips, Glasstic slides can save you money
For easier and faster counting, grid (Neubauer pattern) slides are available.
Capillary action, reproducible precision
Add a drop of solution and a controlled volume of liquid moves into the chamber. The result is a consistent, homogeneous suspension of the sample, ready for microscopic analysis.
Self contained for accuracy and safety
Glasstic slides are manufactured from clear plastic, not glass this reduces the chance of breakage and makes them safer to handle.
Specimens are held in self-contained slide chambers, protecting samples from cross-contamination and leakage onto the lab bench or microscope stage.
“The technicians favoured the use of the KOVA system for several reasons. ... The KOVA system addresses several factors that contribute to imprecision in manual microscopic examinations of body fluids.”
Emerson JF, Emerson SS. Evaluation of a standardized procedure for counting microscopic cells in body fluids. J Clin Lab Anal 2005;19:267-275.
The KOVA System for Urinalysis
Microscopic urinalysis is a powerful diagnostic tool. The HYCOR KOVA System addresses factors that contribute to imprecision in manual microscopic urinalysis. It standardizes and streamlines the sample preparation process, so you reduce variables, improve worker safety and increase the quality and consistency of results.
A Complete System
The KOVA System includes slides, centrifuge tubes, pipetters and dipstick chemistry controls, available with convenient packaging ready to ship from Una Health
1. Right from the start: Take your samples from the collection cup to the centrifuge with KOVA Super Tubes.
2. Separate from the rest: Isolate sediments with the KOVA Pipetter.
3. Easy to see: Use the KOVA Petter to add and mix KOVA stain for visualization of particles
4. Standardized precision: Transfer a drop of each sample to a chamber on the KOVA Glasstic slide for standardized, volume precise microscopic analysis.
Quality control with KOVA-Trol
KOVA Liqua-Trol™ is a ready-to-use liquid control for complete quality control of physical and chemical examination of urine specimens. Liqua-Trol is available with or without microscopics. Liqua-Trol has 30-day room temperature stability.
KOVA-Trol is a stable human urine-based control for physical, chemical and microscopic examination of urine. Use it as an internal quality control to check the performance of test strips and microscopic analysis.
All constituents are stable for 7 days at 2-8°C and 30 days –20°C after reconstitution. KOVA-Trol is available in 3 levels to monitor the entire decision ranges for urine strip chemistries.
KOVA Stain is a modified Sternheimer-Malbin stain that facilitates differentiation and enhances visualization of formed elements in urine sediment.
CALCULATIONS
Undiluted, uncentrifuged Urine or other fluid specimens
Low Particle Count Samples
Count the total cells of a specific type contained in 36 small grids or 4 complete quadrants of the counting grid.
Higher Particle Count Samples
Count the total cells of a specific type contained in 10 small grids in different quadrants of the counting grid.
Alternative Calculation:
Multiply the average number of cells per small grid by 90 to obtain cells per μL; multiply by 90,000 to obtain cells per ml.
Diluted Body Fluids Calculation Method
Cells / μL = Average number of cells per small grid x 90 (multiplication factor) x dilution
e.g., Spinal fluid diluted 1:10; a total of 50 RBC’s counted in 10 small grids
RBC/μL = 50 cells 10 grids x 90 (factor) x 10 (dilution) = 5 x 900 = 4,500 RBC’s/μL
e.g., Semen diluted 1:20; a total of 150 sperm counted in 5 small grids
Sperm/μL = 1550 x 90 (factor) x 20 (dilution) = 30 x 1800 = 54,000 sperm/μL